Mycobacterium tuberculosis molecules by Dr. PI

Acyl carrier proteins (ACPs) are involved in lipid biosynthesis. They shuttle the intermediates between the enzymes that constiute the type II fatty acid synthase system. M. tuberuclosis is unique in producing extremely long fatty acids, the mycolic acids (-> InhA, ). ist the Mycobacterium tuberculosis variant of the acyl carrier protein. It is unique in possessing a longer carboxyl terminus than other ACPs. This contains a phosphopantheteine attachment site, were the 4' phopshphopantetheine is covalently attached through a serine and acts as a swinging arm for the attachment of activated fatty acids and amino acid groups.

spin / | | 3D model needs Jmol

The structural model was obtained from PDB entry , and the description from . Click here to search for at PDB.

is a peptide of with a and a The sequence contains several common . During the reaction, 4'-phosphopantetheine is transferred from CoA to aminoacid residue Ser41 that is covalently binding the phosphopantetheine.

Intragenome comparison reveals two homologous genes in , namely the possible acyl carrier proteins Rv1344 and Rv0033 and to much lower degree polyketide synthase pks12 and the mycobactin mbtE. The search for homologues in reveals amino acid sequence homology to many probable acyl carrier proteins of bacterial and eukaryotic origin. More functional links are available from the .

The gene is encoded in a In the M. tuberculosis laboratory strain H37Rv it is a known as which corresponds to gene in the clinical isolate CDC 1551. It can be found on . The termination codon overlaps with the startcodon of the subsequent gene kasA.

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On the circular M. tuberculosis chromosome lies at .

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blueTB molecules are published by Paul Imboden, Dr. PI Bioconsulting. Authorization to photocopy or reproduce this entry for personal use is granted. Copyright @ 2005 Paul Imboden, Dr. PI Bioconsulting. Last modified May 17, 2008 . Disclaimer: blueTB and the author reserves the right to modify and cancel any statement in these documents and regrets, that he cannot accept any responsibility for the consequences of any such changes. to the best of my knowledge all information is correct, but I cannot accept liability for any errors. References for this blueTB entry are:

Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Cole ST, Brosch R, Parkhill J, Garnier T, Churcher C, Harris D, Gordon SV, Eiglmeier K, Gas S, Barry CE 3rd, Tekaia F, Badcock K, Basham D, Brown D, Chillingworth T, Connor R, Davies R, Devlin K, Feltwell T, Gentles S, Hamlin N, Holroyd S, Hornsby T, Jagels K, Barrell BG, et al. Nature, 393:537-44 (1998) and the Mycobacterium tuberculosis sequencing project from the Sanger Centre.

Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains. Fleischmann RD, Alland D, Eisen JA, Carpenter L, White O, Peterson J, DeBoy R, Dodson R, Gwinn M, Haft D, Hickey E, Kolonay JF, Nelson WC, Umayam LA, Ermolaeva M, Salzberg SL, Delcher A, Utterback T, Weidman J, Khouri H, Gill J, Mikula A, Bishai W, Jacobs Jr WR Jr, Venter JC, Fraser CM. J Bacteriol. 184:5479-90 (2002) and the Mycobacterium tuberculosis sequencing project from TIGR .

3. Dr. PI's Mtbook, The Mycobacterium tuberculosis genome in a book. Paul Imboden, Dr.PI Bioconsulting. mtbook.drpi.ch/ Release 1.8.0 Jan 2004, which itself is based mainly on reference 1.

The solution structure of acyl carrier protein from Mycobacterium tuberculosis. Wong HC, Liu G, Zhang YM, Rock CO, Zheng J. J Biol Chem. 277:15874-15880 (2002).

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