Mycobacterium tuberculosis molecules by Dr. PI

Mycolic acids are major components of the cell wall of Mycobacterium tuberculosis. They have unique functional groups in the acyl chain that are believed to be important for pathogenesis and persistance. These specific modifications of mycolic acids are catalysed by three - or more - mycolic acid cyclopropane synthases PcaA, CmaA2 and CmaA1.

belongs to the cyclopropane-fatty-acyl-phospholipid synthase . It catalyses the following reaction in the :

S-adenosyl-L-methionine
+ phospholipid olefinic fatty acid

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S-adenosyl-L-homocysteine
+ phospholipid cyclopropane fatty acid

spin / | | 3D model needs Jmol

The structural model was obtained from PDB entry , and the description from . Click here to search for at PDB.

is a peptide of with a and a The sequence contains several common , a S-adenosyl-methionine binding motif and two additional SAM-binding regions. is relatively rich in phenylalanine. Cysteine 269 has been found to be essential for catalysis.

Intragenome comparison reveals many highly homologous genes in most of them (methoxy-) mycolic acid methyltransferases or cyclopropane synthases. The search for homologues in identifies close homologues in Mycobacterium leprae. More functional links are available from the .

The gene is encoded in a In the M. tuberculosis laboratory strain H37Rv it is a known as which corresponds to gene in the clinical isolate CDC 1551. It can be found on .

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On the circular M. tuberculosis chromosome lies at .

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blueTB molecules are published by Paul Imboden, Dr. PI Bioconsulting. Authorization to photocopy or reproduce this entry for personal use is granted. Copyright @ 2005 Paul Imboden, Dr. PI Bioconsulting. Last modified May 17, 2008 . Disclaimer: blueTB and the author reserves the right to modify and cancel any statement in these documents and regrets, that he cannot accept any responsibility for the consequences of any such changes. to the best of my knowledge all information is correct, but I cannot accept liability for any errors. References for this blueTB entry are:

Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Cole ST, Brosch R, Parkhill J, Garnier T, Churcher C, Harris D, Gordon SV, Eiglmeier K, Gas S, Barry CE 3rd, Tekaia F, Badcock K, Basham D, Brown D, Chillingworth T, Connor R, Davies R, Devlin K, Feltwell T, Gentles S, Hamlin N, Holroyd S, Hornsby T, Jagels K, Barrell BG, et al. Nature, 393:537-44 (1998) and the Mycobacterium tuberculosis sequencing project from the Sanger Centre.

Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains. Fleischmann RD, Alland D, Eisen JA, Carpenter L, White O, Peterson J, DeBoy R, Dodson R, Gwinn M, Haft D, Hickey E, Kolonay JF, Nelson WC, Umayam LA, Ermolaeva M, Salzberg SL, Delcher A, Utterback T, Weidman J, Khouri H, Gill J, Mikula A, Bishai W, Jacobs Jr WR Jr, Venter JC, Fraser CM. J Bacteriol. 184:5479-90 (2002) and the Mycobacterium tuberculosis sequencing project from TIGR .

3. Dr. PI's Mtbook, The Mycobacterium tuberculosis genome in a book. Paul Imboden, Dr.PI Bioconsulting. mtbook.drpi.ch/ Release 1.8.0 Jan 2004, which itself is based mainly on reference 1.

Crystal structures of mycolic acid cyclopropane synthases from Mycobacterium tuberculosis. Huang CC, Smith CV, Glickman MS, Jacobs WR Jr, Sacchettini JC. J Biol Chem. 277:11559-11569 (2002)

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